beta Actin antibody

Catalog No. ABIN7603831

The Mouse Monoclonal anti-beta Actin antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect beta Actin in samples from Human, Mouse, Rat, Chicken and Rabbit.

Quick Overview for beta Actin antibody (ABIN7603831)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 17A10

Product Details anti-beta Actin Antibody

Purpose: Anti-beta Actin Mouse Monoclonal Antibody

Characteristics: Anti-beta Actin Mouse Monoclonal Antibody (ABIN7603831). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat, Chicken, Rabbit.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human beta Actin

Isotype: IgG1

Application Details

Application Notes: WB 1:3000-1:10000
IHC 1:100-1:200
ICC/IF 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Mouse IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background:

Synonyms: Actin, cytoplasmic 1,Beta-actin,Actin, cytoplasmic 1, N-terminally processed,ACTB,

Tissue Specificity: Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.

Molecular Weight: 42 kDa

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction